As setenta e uma amostras de aguardentes de cana coletadas em alambiques de pequeno e médio porte, provenientes das regiões norte e sul de Minas Gerais, foram avaliadas por CLAE (Cromatografia Líquida de Alta Eficiência) quanto ao teor de acroleína. Avaliaram-se também as concentrações de etanol, acidez volátil e cobre, utilizando-se as metodologias estabelecidas pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Do total de amostras, 9,85% apresentaram níveis de acroleína acima do limite legal, 21,00% apresentaram teores de cobre e 8,85% de acidez volátil acima dos limites estabelecidos pela legislação brasileira. Os teores de acroleína variaram de 0 a 21,97 mg.100 mL-1 de etanol. Entretanto, não ocorreu diferença significativa no nível de 5% de significância entre as amostras do norte e as do sul de Minas Gerais. O método aplicado para a determinação de acroleína em aguardente de cana foi a derivação com 2,4-dinitrofenilidrazina (DNPH) e a posterior análise por CLAE.

Seventy-one samples of sugarcane spirits from small and average size stills produced in the northern and southern Minas Gerais (Brazil) were analyzed for acrolein using HPLC (High Performance Liquid Chromatography). Ethanol and copper concentrations and volatile acidity were also determined according to methods established by the Ministry of Agriculture, Livestock and Supply (MAPA). A total of 9.85% of the samples tested showed levels of acrolein above the legal limits, while the copper concentrations of 21.00% of the samples and the volatile acidity of 8.85% of the samples were higher than the limits established by the Brazilian legislation. The concentration of acrolein varied from 0 to 21.97 mg.100 mL-1 of ethanol. However, no significant difference at 5% of significance was observed between the samples produced in the northern and southern Minas Gerais. The method used for determination of acrolein in sugarcane spirits involved the formation of a derivative with 2,4-dinitrophenylhydrazine (2,4-DNPH) and subsequent analysis by HPLC.

In this study, we evaluated the yield and chemical composition of volatile compounds obtained from fresh, dried at 30 and 40 ºC respectively of Piper piscatorum, employing the technique of hydrodistillation. The volatile were chemically characterized by GC-MS and GC-FID. The main volatile compounds were selin-11-en-4-a-ol (57,63 ± 3,07%; 53,95 ± 1,56% and 56,20 ± 0,43%) and benzyl benzoate (15,40 ± 0,36%; 16,32 ± 0,85% and 15,04 ± 0,24%).The drying of the raw material reduced yield of volatile compounds, changing their chemical compositions.

The objective of the present study was the evaluation of the presence of organic and inorganic contaminants in samples of aged cachaça from the South of the state of Minas Gerais. Furfural, methanol and copper were determined by colorimetric reactions, while the analyses of ethyl carbamate and acrolein were performed by GC/MS and HPLC, respectively. High levels of furfural and copper were obtained. All samples showed concentrations below the established by legislation for the ethyl carbamate, and for acrolein, only one sample showed higher levels. Methanol was not detected in the samples.

The objective of the present study was the identification and quantification of ethyl carbamate (EC), by HPLC-FLD, after different periods of storage in an oak (Quercus sp) barrel and a glass vessel. The concentration of EC in the cachaça samples varied from <LD to 17.46 µg L-1, for the period during which the cachaça was stored in an oak barrel, and <LQ to 18.51 µg L-1 for the cachaça stored in a glass recipient. The storage of the beverage in both the oak barrel and the glass vessel resulted in a significant increase in the concentration of this contaminant.

O objetivo deste trabalho foi avaliar a atividade fungicida in vitro do óleo essencial das folhas de Piper hispidinervum sobre Bipolaris sorokiniana, Fusarium oxysporum e Colletotrichum gloeosporioides. Para os ensaios biológicos, empregou-se o teste bioanalítico in vitro utilizando as concentrações de 100, 200, 500, 1000, 1500 e 2000 µg.mL-1 do óleo essencial. Estas foram incorporadas no meio de cultura BDA (batata-dextrose-ágar) para avaliação do crescimento ou inibição micelial. O delineamento estatístico utilizado foi o inteiramente casualizado, com quatro repetições. Na concentração de 200 µg.mL-1, observou-se uma inibição total do fitopatógeno Bipolaris sorokiniana enquanto que, para o Fusarium oxysporum e o Colletotrichum gloeosporioides esta ocorreu na concentração de 1000 µg.mL-1.

The objective of this study was to evaluate the in vitro antifungal activity of the essential oil of the leaves of Piper hispidinervum against Bipolaris sorokiniana, Fusarium oxysporum and Colletotrichum gloeosporioides. For the biological tests, using the bioanalitic test in vitro the concentrations of 100, 200, 500, 1000, 1500 and 2000 µg.mL-1 the essential oil. This were incorporated into PDA (potato dextrose agar) medium in order to evaluate fungal mycelial growth or inhibition. The statistic design used was completely randomized, with four replicates. In the concentration of 200 µg.mL-1, observed inhibited complete the phytopathogens Bipolaris sorokiniana while the Fusarium oxysporum and Colletotrichum gloeosporioides this is occurred in the concentration of 1000 µg.mL-1.